Evaluation of a Latex Agglutination Method for Detecting and Characterizing Verotoxin (VT) Produced by Escherichia coli
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چکیده
منابع مشابه
Evaluation of a microplate latex agglutination method (Verotox-F assay) for detecting and characterizing verotoxins (Shiga toxins) in Escherichia coli.
The performance of a commercial microplate latex agglutination assay, the Verotox-F assay, was compared with that of the Vero cell assay for the detection and characterization of Escherichia coli verocytotoxins (VTs). Culture filtrates of 68 VT-positive E. coli strains (65 human isolates [33 of serotype O157:H7/H-, 32 of non-O157 serotypes] and 3 reference strains) and 104 VT-negative strains (...
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The value of a latex agglutination test (Escherichia coli O157 latex test; Oxoid Ltd.) for rapid presumptive detection of E. coli serotype O157:H7 was determined by laboratory trials and during an outbreak of hemorrhagic colitis. The latex test was found to be a simple, highly efficient and reliable test in detecting E. coli O157:H7 with 100% sensitivity and specificity. It was also found that ...
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in this study which has been carried out from 1996 until 1997 soluble latex agglutination antigen was prepared from L, donovani,sudan(s-1) by culturing in special media,harvesting,and later solubilizing by freezing and thawing.the antigen were futher prepared by high speed centrifugation.after measurement of the protein (lowry method) concentration of the antigen was set at 2.2 mg/ml,the antige...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 1997
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.71.248